Chiralpak ad-h pdf

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INSTRUCTION MANUAL FOR CHIRALPAK® AY-H Please read this instruction sheet completely before using this column 800 North Five Points Road West Chester, PA 19380 Tel: 610-594-2100 Fax: 610-594-2325 chiral@chiraltech.com chiraltech.com . 07/2013LE Page 2 Operating Procedure Please contact Chiral Technologies for further assistance before trying any solvents not mentioned below. A – Mobile
CHIRALPAK ® AD-RH, AS-RH & CHIRALCEL ® OD-RH, OJ-RH COLUMNS. 8 2.1 x 150 mm Analytical column 4.6 x 150 mm Analytical column Flow rate direction As indicated on the column label Typical Flow rate ~ 0.1 to .3ml/min ~ 0.5 to 1.0ml/min Pressure limitation Should be maintained < 300 Bar (4350 psi) for maximum column life Adapt flow rates to column size. pH Between pH 2.0 and pH 9.0 Temperature : CHIRALPAK® AD-H 0.46cmΦ × 25cmL (L51): .8mL/min. Signal-to-noise ratio ≧10 29.7 For details of monograph, please check pharmacopeia. Requirement Result Resolution ≧1.5 3.0 0 10 20 30 Time (min) 0 2 4 6 8 10 t 5 System suitability System suitability solution: 8 mg/mL of USP Sitagliptin System suitability Mixture RS in Diluent

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